Coding
Part:BBa_K1114508:Design
Designed by: Jake Awtry Group: iGEM13_BostonU (2013-09-12)
Level 1 MoClo device with lacI and YFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
pR40B2Y_FG This is a Level 1 MoClo device that expresses YFP under the repressible promoter lacI. It contains the Level 0 parts R0040_FB, BCD2_BC, E0030_CD, and B0015_DG within the DVL1_EF backbone. In the given sequence the internal fusion sites are incorrectly duplicated. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.
Source
Construct of MoClo Level 0 parts derived from iGEM Distribution Kits